Effectiveness of Different Cleaning Agents against the Colonization of Candida spp and the in Vitro Detection of the Adherence of These Yeast Cells to Denture Acrylic Surfaces

PURPOSE: The aim of this study is to examine the effect Klorhex and Fittydent, which are used as cleaning agents on the adhesion of Candida on the surfaces of acrylic denture and palatal mucosa. In addition, ability of yeasts to adhere to acrylic strips was evaluated after applying these agents in vitro. MATERIALS and METHODS: Each group of 15 patients cleaned their dentures with either Klorhex or with Fittydent. The control group cleaned their dentures with water. RESULTS: It was found that 62.2% of the patients had colonies of Candida species on their palatal mucosa which was reduced to 51.1% after using these cleaning agents. The colonization rate with Candida spp on their dentures was reduces from 82.2% to 68.8% using these cleaning agents. The mean adhesion value of the Candida strains isolated from the acrylic strips were found to be 75cell/strip prior to applying the Klorhex and Fittydent and 37.5cell/strip and 15cell/strip after applying these agents, respectively. CONCLUSION: These results showed that Klorhex and Fittydent have a certain preventive effect on the colonization rate of Candida spp on the surface of these dentures, the palatal mucosa, as well as on the acrylic strips in vitro.

Evaluation of the disc diffusion method with a comparison study for fluconazole susceptibility of Candida strains / 中华医学杂志(英文版)

<p><b>OBJECTIVE</b>To performance susceptibility testing of antifungal agents. Due to the increasing number of resistant strains, susceptibility testing of antifungal agents is gaining importance.</p><p><b>METHODS</b>We compared the results of standard macrotube dilution reference method with two different microdilution methods, as well as the disc diffusion method in order to test the susceptibility of 150 Candida strains to fluconazole.</p><p><b>RESULTS</b>Overall correlation between microdilution and macrodilution methods was 86%. It was 91% between the Minimal Inhibitory Concentrations obtained from macrodilution and disc diffusion zone diameters.</p><p><b>CONCLUSION</b>The disc diffusion test was evaluated as a low-cost, reproducible, and efficient way of assessing the in vitro susceptibility of Candida strains to fluconazole.</p>